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ML-06 Diagnostic value of liquid biopsy for CNS lymphoma by detection of specific gene mutations in the cerebrospinal fluid by Yuki Yamagishi; Nobuyoshi Sasaki; Yuko Matsushita; Saki Shimizu; Kuniaki Saito; Keiichi Kobayashi; Yoshiaki Shiokawa; Motoo Nagane; Koichi Ichimura is a Medicine article available to read on EtoBox.
What is ML-06 Diagnostic value of liquid biopsy for CNS lymphoma by detection of specific gene mutations in the cerebrospinal fluid about?
## Abstract Backgrounds & Purpose: Central nervous system lymphoma (CNSL) is the second most common primary malignant brain tumor. Brain biopsy is indispensable to confirm the diagnosis of CNSL, but has a potential risk of inducing hemorrhagic complications in the brain. Therefore, liquid biopsy using the cerebrospinal fluid (CSF) has attracted an attention as a less invasive diagnostic method. In this study, we established a digital PCR-based method to detect __MYD88__ mutations in CSF and evaluated its efficacy. Methods: Matched CSF and biopsy samples from CNSL patients collected before the start of chemotherapy were used. Cellular DNA and cell free DNA (cfDNA) of CSF were separately extracted from the pellet and the supernatant fraction of CSF, respectively. Presence of the __MYD88__ L265P mutation was examined in each fraction by the digital PCR. The mutational status obtained by liquid biopsy was compared with that of the matched biopsy specimen examined by pyrosequencing. Result: A total of 36 paired samples were used. When the cutoff value of Target/Total ratio was 0.25%, sensitivity, specificity, and area under the curve (AUC) of the digital PCR detection using cellular DNA
Who reads ML-06 Diagnostic value of liquid biopsy for CNS lymphoma by detection of specific gene mutations in the cerebrospinal fluid?
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- Author
- Yuki Yamagishi; Nobuyoshi Sasaki; Yuko Matsushita; Saki Shimizu; Kuniaki Saito; Keiichi Kobayashi; Yoshiaki Shiokawa; Motoo Nagane; Koichi Ichimura
- Publisher
- Oxford University Press (OUP)
- Published
- 2020
- Language
- EN
- Field
- Medicine (Health Sciences)