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Viral and Murine Interleukin-10 Are Correctly Processed and Retain Their Biological Activity When Produced in Tobacco by Luisa Bortesi; Marzia Rossato; Flora Schuster; Nicole Raven; Johannes Stadlmann; Linda Avesani; Alberto Falorni; Flavia Bazzoni; Ralph Bock; Stefan Schillberg; Mario Pezzotti is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Viral and Murine Interleukin-10 Are Correctly Processed and Retain Their Biological Activity When Produced in Tobacco about?
## Abstract ## Background Interleukin-10 (IL-10) is a potent anti-inflammatory cytokine, with therapeutic applications in several autoimmune and inflammatory diseases. Oral administration of this cytokine alone, or in combination with disease-associated autoantigens could confer protection form the onset of a specific autoimmune disease through the induction of oral tolerance. Transgenic plants are attractive systems for production of therapeutic proteins because of the ability to do large scale-up at low cost, and the low maintenance requirements. They are highly amenable to oral administration and could become effective delivery systems without extensive protein purification. We investigated the ability of tobacco plants to produce high levels of biologically-active viral and murine IL-10. ## Results Three different subcellular targeting strategies were assessed in transient expression experiments, and stable transgenic tobacco plants were generated with the constructs that yielded the highest accumulation levels by targeting the recombinant proteins to the endoplasmic reticulum. The best yields using this strategy in T~1~plants were 10.8 and 37.0 μg/g fresh leaf weight for viral
Who reads Viral and Murine Interleukin-10 Are Correctly Processed and Retain Their Biological Activity When Produced in Tobacco?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Luisa Bortesi; Marzia Rossato; Flora Schuster; Nicole Raven; Johannes Stadlmann; Linda Avesani; Alberto Falorni; Flavia Bazzoni; Ralph Bock; Stefan Schillberg; Mario Pezzotti
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, 2001-; Springer Science and Business Media LLC (ISSN 1472-6750)
- Published
- 2009
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)