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Simple High-performance Liquid Chromatographic Determination of the Protease Inhibitor Indinavir in Human Plasma by Anura L Jayewardene; Frank Zhu; Francesca T Aweeka; John G Gambertoglio is a Chemistry article available to read on EtoBox.

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Indinavir is a member of a class of protease inhibitors that actively prevent the acquired immunodeficiency syndrome virion from maturing. A high-performance liquid chromatographic (HPLC) assay was developed and validated for the determination of indinavir in human plasma. Indinavir and the internal standard were isolated from the plasma by ether extraction. The residue after evaporation of ether was reconstituted with buffer and injected onto a C reversed-phase column 4 eluted isocratically with a mobile phase consisting of 35:65 (v / v) of acetonitrile and buffer. A wavelength of 210 nm was found to be optimum for detection. The calibration range of this assay was from 10 to 5000 ng / ml and coefficients of variation for the assay ranged from 4.6% to 11.0% for three different drug concentrations and the limit of quantitation was 10 ng / ml. During the validation, short-term stability of the drug in plasma, stability during heat deactivation and on repeated freezing and thawing of plasma was evaluated. The overall recovery of indinavir by the ether extraction method was 91.4%. This HPLC assay was found to be a simple and reproducible method for monitoring indinavir levels in human

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Author

Anura L Jayewardene; Frank Zhu; Francesca T Aweeka; John G Gambertoglio

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-4347)

Published

1998

Language

EN

Field

Chemistry (Physical Sciences)