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Cyclodextrins as a Useful Tool for Bioconversions in Plant Cell Biotechnology by Wim van Uden; Herman J. Woerdenbag; Niesko Pras is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The application of cyclodextrins as precursor solubilizers in biotechnological processes, in which plant cells are involved, is new. In this paper the possibilities for cyclodextrin facilitated bioconversions by freely suspended and/or immobilized plant cells or plant enzymes are demonstrated. After complexation with/~-cyclodextrin, the phenolic steroid 17/~-estradiol could be ortho-hydroxylated into a catechol, mainly 4-hydroxyestradiol, by a phenoloxidase from in vitro grown cells of Mucuna pruriens. By complexation with/3-cyclodextrin the solubility of the steroid increased from almost insoluble to 660/~.M. In addition, by complexation with/~-cyclodextdn, a solution of 3 mM coniferyl alcohol could be fed to cell cultures of Podophyllum hexandrum in order to enhance the accumulation of podophyllotoxin. Finally, the glucosylation of podophyllotoxin by cell cultures derived from Linumflavum was investigated. Four cyclodextrins:/3-cyclodextrin, 7-cyclodextrin, hydroxypropyl-/3-cyclodextrin and dimethyl-flcyclodextrin were used to improve the solubility of podophyllotoxin. Dimethyl-/3-cyclodextrin met our needs the best and the solubility of podophyUotoxin could be enhanced from 0.15

Who reads Cyclodextrins as a Useful Tool for Bioconversions in Plant Cell Biotechnology?

Author

Wim van Uden; Herman J. Woerdenbag; Niesko Pras

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC (ISSN 0167-6857)

Published

1994

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)