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Guanidine hydrochloride extraction and detection of prion proteins in mouse and hamster prion diseases by ELISA by Shin-Chung Kang; Ruliang Li; Chuanping Wang; Tao Pan; Tong Liu; Richard Rubenstein; Geoff Barnard; Boon-Seng Wong; Man-Sun Sy is a Medicine article available to read on EtoBox.
What is Guanidine hydrochloride extraction and detection of prion proteins in mouse and hamster prion diseases by ELISA about?
## Abstract Current detection of transmissible spongiform encephalopathy (TSE) relies on the proteolytic generation of a protease‐resistant core from the scrapie isoform of prion protein (PrP^Sc^) followed by immunoblotting. This process is non‐quantitative, time‐consuming, and technically demanding. Recently, an alternative __in vitro__ test for TSE based on the differential extraction of brain homogenates using guanidine hydrochloride followed by DELFIA (Dissociation Enhanced Lanthanide FluoroImmunoAssay) has been developed. In the present study, this approach was adopted using a panel of anti‐PrP monoclonal antibodies (MAbs) in conventional sandwich enzyme‐linked immunosorbent assay (ELISA) to investigate hamster and two distinct strains of mouse prion diseases. Although PrP species were present in both soluble and insoluble fractions from normal as well as TSE samples, only the PrP species in the insoluble fractions from the latter samples were protease‐resistant. In addition, certain anti‐PrP MAb pairs could distinguish the PrP species in infected brains from those in the normal samples. The ability to differentiate disease‐associated PrP isoforms without proteinase K digestio
Who reads Guanidine hydrochloride extraction and detection of prion proteins in mouse and hamster prion diseases by ELISA?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Shin-Chung Kang; Ruliang Li; Chuanping Wang; Tao Pan; Tong Liu; Richard Rubenstein; Geoff Barnard; Boon-Seng Wong; Man-Sun Sy
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-3417)
- Published
- 2003
- Language
- EN
- Field
- Medicine (Health Sciences)