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Can I read Laccase immobilized on chitosan-polyacrylic acid microspheres as highly efficient biocatalyst for naphthol green B and indigo carmine degradation on EtoBox?
Laccase immobilized on chitosan-polyacrylic acid microspheres as highly efficient biocatalyst for naphthol green B and indigo carmine degradation by Anca Ruxandra Leontieș; Adina Răducan; Daniela Cristina Culiță; Elvira Alexandrescu; Alina Moroșan; Dan Eduard Mihaiescu; Ludmila Aricov is a Engineering article available to read on EtoBox.
What is Laccase immobilized on chitosan-polyacrylic acid microspheres as highly efficient biocatalyst for naphthol green B and indigo carmine degradation about?
Low cost and non-toxic microspheres constituted by different ratios of chitosan (Cs) and polyacrylic acid (PAA) were manufactured as carriers for laccase (Lc). The CsPAA microspheres characterization proved that the PAA content played an important role on the size, surface morphology and rheological properties, and modulated the enzyme immobilization. The immobilization of Lc involved a multi-step procedure in which the 1-ethyl-3-(3dimethylaminopropyl carbodiimide hydrochloride (EDC) was used as "zero length" crosslinker. The product with the largest active enzyme amount (44.38 mg Lc/g dry support), and the best operational stability profile was 10CsPAALc 3 . Also, this product contained the lowest PAA concentration and had excellent rheological properties. Moreover, two current hazardous chemicals, indigo carmine (IC) and naphthol green B (NG), were used as model dyes in practical applications. The biocatalytic decomposition of NG and IC was 1.67 and 2.4 times faster for 10CsPAALc 3 compared to the free enzyme. The kinetic profile revealed that the immobilized Lc was less prone to substrate, operational and thermal inactivation. For example, the thermal inactivation for 10CsPAALc
Who reads Laccase immobilized on chitosan-polyacrylic acid microspheres as highly efficient biocatalyst for naphthol green B and indigo carmine degradation?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Anca Ruxandra Leontieș; Adina Răducan; Daniela Cristina Culiță; Elvira Alexandrescu; Alina Moroșan; Dan Eduard Mihaiescu; Ludmila Aricov
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Engineering (Physical Sciences)