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Impacts of combined water-saving irrigation and controlled-release urea on CH4 emission and its associated microbial comm... 1 1. Introduction 2 2. Materials and methods 2 2.1. Experiment site and soil characteristics 2 2.2. Experimental design 2 2.3. CH4 gas sampling and measurement 3 2.4. Soil sampling and measurement 3 2.4.1. Soil enzyme activity and organic acid 4 2.4.2. DNA extraction, shotgun sequencing and metagenomic analysis 4 2.4.2.1. DNA extraction 4 2.4.2.2. Shotgun sequencing 4 2.4.2.3. Metagenomic analysis 4 2.5. Statistical analysis 4 3. Results 4 3.1. CH4 emissions during rice growing season 4 3.2. Soil enzyme activity and organic acid content 5 3.3. Structure and activities of methanogens and methanotrophs 6 3.4. The distribution of core methanogen species and key pathway 6 3.5. Correlation analysis between cumulative CH4 emission, soil enzyme activity, organic acid and microbial communities and function 7 4. Discussion 8 4.1. Mitigation of CH4 emission by water-saving irrigation and controlled-release urea 8 4.2. Water-saving irrigation mode and controlled-release urea deeply shape the microbiota communities in paddy soil 10 4.3. Impacts of irrigation mode and n

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Language

UND

Category

nonfiction