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Rim 2/Hipa CACTA transposon display ; A new genetic marker technique in Oryza species by Soon-Jae Kwon; Kyong-Chul Park; Jin-Hong Kim; Ju Kyong Lee; Nam-Soo Kim is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Background Transposons constitute the major fractions of repetitive sequences in eukaryotes, and have been crucial in the shaping of current genomes. Transposons are generally divided into two classes according to the mechanism underlying their transposition: RNA intermediate class 1 and DNA intermediate class 2. CACTA is a class 2 transposon superfamily, which is found exclusively in plants. As some transposons, including the CACTA superfamily, are highly abundant in plant species, and their nucleotide sequences are highly conserved within a family, they can be utilized as genetic markers, using a slightly modified version of the conventional AFLP protocol. __Rim2 /Hipa__ is a CACTA transposon family having 16 bp consensus TIR sequences to be present in high copy numbers in rice genome. This research was carried out in order to develop a __Rim2/Hipa__ CACTA-AFLP or __Rim2/Hipa__ CACTA-TD (transposon display, hereafter __Rim2/Hipa__-TD) protocol for the study of genetic markers in map construction and the study of genetic diversity in rice. ## Results __Rim2/Hipa__-TD generated ample polymorphic profiles among the different rice accessions, and the amplification profiles were hi

Who reads Rim 2/Hipa CACTA transposon display ; A new genetic marker technique in Oryza species?

Author

Soon-Jae Kwon; Kyong-Chul Park; Jin-Hong Kim; Ju Kyong Lee; Nam-Soo Kim

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2000-; Springer Science and Business Media LLC (ISSN 1471-2156)

Published

2005

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)