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Can I read GlcNAc6ST-1-mediated decoration of MAdCAM-1 protein with L-selectin ligand carbohydrates directs disease activity of ulcerative colitis on EtoBox?
GlcNAc6ST-1-mediated decoration of MAdCAM-1 protein with L-selectin ligand carbohydrates directs disease activity of ulcerative colitis by Motohiro Kobayashi; Hitomi Hoshino; Junya Masumoto; Mana Fukushima; Kenichi Suzawa; Shunsuke Kageyama; Manabu Suzuki; Haruo Ohtani; Minoru Fukuda; Jun Nakayama is a Medicine article available to read on EtoBox.
What is GlcNAc6ST-1-mediated decoration of MAdCAM-1 protein with L-selectin ligand carbohydrates directs disease activity of ulcerative colitis about?
## Background: A diffuse lymphocyte infiltrate is 1 of the characteristic features of ulcerative colitis (uc). such lymphocyte recruitment requires lymphocyte rolling mediated by l-selectin ligand carbohydrates (6-sulfo sialyl lewis x-capped o-glycans) and/or mucosal addressin cell adhesion molecule 1 (madcam-1) expressed on high endothelial venule (hev)-like vessels. the present study was undertaken to elucidate the role of madcam-1 posttranslationally modified ("decorated") with l-selectin ligand carbohydrates in uc pathogenesis and consequent clinical outcomes. ## Methods: Biopsy specimens composed of active and remission phases of uc as well as normal colonic mucosa were immunostained for cd34, madcam-1, and meca-79, and the immunostained sections were quantitatively analyzed. reverse-transcriptase polymerase chain reaction (rt-pcr) was carried out to evaluate transcripts of madcam-1 and n-acetylglucosamine-6-o-sulfotransferases (glcnac6sts). cho and lec2 cells transfected with cd34 and madcam-1 together with enzymes involved in l-selectin ligand carbohydrate biosynthesis were analyzed by immunofluorescence, facs, and western blotting to characterize the biochemical properties
Who reads GlcNAc6ST-1-mediated decoration of MAdCAM-1 protein with L-selectin ligand carbohydrates directs disease activity of ulcerative colitis?
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- Author
- Motohiro Kobayashi; Hitomi Hoshino; Junya Masumoto; Mana Fukushima; Kenichi Suzawa; Shunsuke Kageyama; Manabu Suzuki; Haruo Ohtani; Minoru Fukuda; Jun Nakayama
- Publisher
- John Wiley and Sons; Oxford University Press; Oxford University Press (OUP) (ISSN 1078-0998)
- Published
- 2009
- Language
- EN
- Field
- Medicine (Health Sciences)