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The Effect of Micropores in the Surface of Temperature-responsive Culture Inserts on the Fabrication of Transplantable Canine Oral Mucosal Epithelial Cell Sheets by Daisuke Murakami; Masayuki Yamato; Kohji Nishida; Takeshi Ohki; Ryo Takagi; Joseph Yang; Hideo Namiki; Teruo Okano is a Engineering article available to read on EtoBox.

What is The Effect of Micropores in the Surface of Temperature-responsive Culture Inserts on the Fabrication of Transplantable Canine Oral Mucosal Epithelial Cell Sheets about?

Primary canine oral mucosal epithelial cells were cultured on temperature-responsive dishes and cell culture inserts to fabricate transplantable epithelial cell sheets. When 3T3 feeder layers and fetal bovine serum were eliminated from dish culture, the harvested cell sheets became significantly more fragile. In contrast, when epithelial cells were cultured on inserts having submicron-scale pores, cell sheet fragility was eliminated. Keratin expression profiles showed no differences among the harvested cell sheets, but the expression of p63, a putative stem/progenitor marker, was strongly dependent on the presence of 3T3 feeder layers and serum. These results suggest that the maintenance of stem/progenitor cells is influenced by the apical/basal supply of nutrients as well as culture supplements.

Who reads The Effect of Micropores in the Surface of Temperature-responsive Culture Inserts on the Fabrication of Transplantable Canine Oral Mucosal Epithelial Cell Sheets?

Author

Daisuke Murakami; Masayuki Yamato; Kohji Nishida; Takeshi Ohki; Ryo Takagi; Joseph Yang; Hideo Namiki; Teruo Okano

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0142-9612)

Published

2006

Language

EN

Field

Engineering (Physical Sciences)