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Development and evaluation of an enzyme-linked immunosorbent assay to detect Pieris rapae remains in guts of arthropod predators by M.A. Schmaedick; K.S. Ling; D. Gonsalves; A.M. Shelton is a Agricultural and Biological Sciences article available to read on EtoBox.

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## Abstract An enzyme‐linked immunosorbent assay (ELISA) was developed to detect remains of __Pieris rapae__ L. (Lepidoptera: Pieridae) immature stages in the guts of field collected arthropod predators. The assay can be used to help ascertain the relative importance of arthropod predator species in suppressing __P. rapae__ in cabbage, __Brassica oleracea__ var. __capitata__ L. The ELISA is sensitive to all immature stages of __P. rapae__, although first and fifth instars can be detected more readily than eggs or pupae and third instars showed intermediate detectability. Assays on whole body homogenates of predators readily detected predation on __P. rapae__ first instars by all seven of the predator species tested, although response generally declined with increasing predator size. Together the results show that the __P. rapae__ ELISA possesses a sufficiently high level of sensitivity and specificity to be a useful tool in helping to elucidate the roles of arthropod predator species in reducing populations of __P. rapae__ in cabbage.

Who reads Development and evaluation of an enzyme-linked immunosorbent assay to detect Pieris rapae remains in guts of arthropod predators?

Author

M.A. Schmaedick; K.S. Ling; D. Gonsalves; A.M. Shelton

Publisher

Springer; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0013-8703)

Published

2001

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)