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No evidence of the Shiga toxin-producing E. coli O104:H4 outbreak strain or enteroaggregative E. coli (EAEC) found in cattle faeces in northern Germany, the hotspot of the 2011 HUS outbreak area by Lothar H Wieler; Torsten Semmler; Inga Eichhorn; Esther M Antao; Bianca Kinnemann; Lutz Geue; Helge Karch; Sebastian Guenther; Astrid Bethe is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is No evidence of the Shiga toxin-producing E. coli O104:H4 outbreak strain or enteroaggregative E. coli (EAEC) found in cattle faeces in northern Germany, the hotspot of the 2011 HUS outbreak area about?

## Abstract ## Background Ruminants, in particular bovines, are the primary reservoir of Shiga toxin-producing __E. coli__ (STEC), but whole genome analyses of the current German ESBL-producing O104:H4 outbreak strain of sequence type (ST) 678 showed this strain to be highly similar to enteroaggregative __E. coli__ (EAEC). Strains of the EAEC pathotype are basically adapted to the human host. To clarify whether in contrast to this paradigm, the O104:H4 outbreak strain and/or EAEC may also be able to colonize ruminants, we screened a total of 2.000 colonies from faecal samples of 100 cattle from 34 different farms - all located in the HUS outbreak region of Northern Germany - for genes associated with the O104:H4 HUS outbreak strain (__stx2__, __terD__, __rfb__ ~O104~, __fliC__ ~H4~), STEC (__stx1__, __stx2__, __escV__), EAEC (__pAA__, __aggR, astA__), and ESBL-production (__bla__ ~CTX-M~, __bla__ ~TEM~, __bla__ ~SHV~). ## Results The faecal samples contained neither the HUS outbreak strain nor any EAEC. As the current outbreak strain belongs to ST678 and displays an en-teroaggregative and ESBL-producing phenotype, we additionally screened selected strains for ST678 as well as the a

Who reads No evidence of the Shiga toxin-producing E. coli O104:H4 outbreak strain or enteroaggregative E. coli (EAEC) found in cattle faeces in northern Germany, the hotspot of the 2011 HUS outbreak area?

Author

Lothar H Wieler; Torsten Semmler; Inga Eichhorn; Esther M Antao; Bianca Kinnemann; Lutz Geue; Helge Karch; Sebastian Guenther; Astrid Bethe

Publisher

BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, 2009-; Springer Science and Business Media LLC (ISSN 1757-4749)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)