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Cultivatable microbial biodiversity: gnawing at the Gordian knot by Brian J. Tindall; Evelyne Brambilla; Maike Steffen; Regine Neumann; Rüdiger Pukall; Reiner M. Kroppenstedt; Erko Stackebrandt is a Environmental Science article available to read on EtoBox.

What is Cultivatable microbial biodiversity: gnawing at the Gordian knot about?

Rapid and inexpensive sorting of bacterial isolates may be achieved using Fourier transform infrared spectroscopy (FT‐IR), a method that has hitherto been applied to identification and classification. The comprehensive characterization of environmental samples requires the isolation of large numbers of isolates using different growth media and growth conditions. In such cases, sorting the isolates is critical before isolates are subjected to more detailed studies. Using FT‐IR, isolates are grown under standardized conditions, and 100 strains can be tested within less than 8 h. Chemotaxonomic and molecular characterization of members of clusters emerging from FT‐IR analysis either at a level of spectral distance values below 20–30 (analysis of region 600–800 cm^−1^, average linkage algorithm) or at spectral heterogeneity values below 75 (regions 1200–900, 3000–2798 and 901–698, scaling to first region, Ward's algorithm) reveals great similarities in fatty acids and 16S rDNA sequences. As judged from riboprinting analyses and fatty acid analyses, FT‐IR analysis is able to unravel intraspecific subclustering. The example used in this study of 100 isolates from a mat system, Lake Fryxe

Who reads Cultivatable microbial biodiversity: gnawing at the Gordian knot?

Author

Brian J. Tindall; Evelyne Brambilla; Maike Steffen; Regine Neumann; Rüdiger Pukall; Reiner M. Kroppenstedt; Erko Stackebrandt

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 1462-2912)

Published

2000

Language

EN

Field

Environmental Science (Physical Sciences)