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Vertebrate Primary Cilia: a Sensory Part of Centrosomal Complex in Tissue Cells, but a “Sleeping Beauty” in Cultured Cells? by Irina B. Alieva; Ivan A Vorobjev is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Primary cilium development along with other components of the centrosome in mammalian cells was analysed ultrastructurally and by immunofluorescent staining with anti‐acetylated tubulin antibodies. We categorized two types of primary cilia, nascent cilia that are about 1 μm long located inside the cytoplasm, and true primary cilia that are several μm long and protrude from the plasma membrane. The primary cilium is invariably associated with the older centriole of each diplosome, having appendages at the distal end and pericentriolar satellites with cytoplasmic microtubules emanating from them. Only one cilium per cell is formed normally through G~0~, S and G~2~phases. However, in some mouse embryo fibroblasts with two mature centrioles, bicilates were seen. Primary cilia were not observed in cultured cells where the mature centriole had no satellites and appendages (Chinese hamster kidney cells, line 237, some clones of l‐fibroblasts). In contrast to primary cilia, striated rootlets were found around active and non‐active centrioles with the same frequency. In proliferating cultured cells, a primary cilium can be formed several hours after mitosis, in fibroblasts 2–4 h

Who reads Vertebrate Primary Cilia: a Sensory Part of Centrosomal Complex in Tissue Cells, but a “Sleeping Beauty” in Cultured Cells??

Author

Irina B. Alieva; Ivan A Vorobjev

Publisher

Elsevier Science; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 1065-6995)

Published

2004

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)