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Ligand-Receptor Interactions in the Membrane of Cultured Cells Monitored by Fluorescence Correlation Spectroscopy by Aladdin Pramanik; Rudolf Rigler is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Ligand-Receptor Interactions in the Membrane of Cultured Cells Monitored by Fluorescence Correlation Spectroscopy peptides, hormones, natural products) and their different receptor subtypes are, therefore, often overlooked by the conventional equilibrium binding technique. In fluorescence correlation spectroscopy (FCS) the small laser volume element (0.2 fl) allows the detection of single molecules (Rigler, 1995) as well as the measurement of molecular properties at specific coordinates in the cell membrane (Rigler et al., 1999). With single molecule detection sensitivity FCS allows the detection of the interaction of ligand with binding sites of receptors on the molecular level in their native environment on cell surfaces (Rigler et al., 1999;Pramanik et al., 1999;Boonen et al., 2000). This technique permits the identification of receptors or target molecules which was not possible before by other conventional binding techniques, especially by isotope labeling. Very recently, we were able to demonstrate the proinsulin C-peptide binding in protein extracts, which was not possible to detect before (Henriksson et al., 2001). FCS also allows the study of apoptosis; we have recently

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Author

Aladdin Pramanik; Rudolf Rigler

Publisher

Walter de Gruyter GmbH

Published

2001

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)