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Molecular differentiation in pea powdery-mildew haustoria: Identification of a 62-kDa N-linked glycoprotein unique to the haustorial plasma membrane by Andrew J. Mackie; Angela M. Roberts; James A. Callow; Jonathan R. Green is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Molecular differentiation in pea powdery-mildew haustoria: Identification of a 62-kDa N-linked glycoprotein unique to the haustorial plasma membrane about?

Monoclonal antibodies have been raised against haustorial complexes isolated from pea (Pisum sativum L.) leaves infected by the biotrophic powdery mildew fungus Erysiphe pisi D.C. Immuno-localisation studies, using isolated haustorial complexes and infected pea leaf material, have shown that one of the antibodies, designated UB7, binds to fungal wall and plasma membranes present in both haustoria and mycelia. However, a second antibody, UB8, binds specifically to the haustorial plasma membrane, and does not label fungal plasma membranes in mycelia. Western blotting and antigenmodification techniques have shown that UB8 recognises a protein epitope of a 62-kDa antigen. A reduction in molecular weight of this component after endo-F treatment indicates that the antigen is an N-linked glycoprotein. UB7 also recognises a 62-kDa glycoprotein, which is susceptible to endo-F treatment, and the antibody binds to a carbohydrate epitope. Differences in molecular weights of the products after endo-F treatment of antigens show that the 62-kDa glycoproteins recognised by the antibodies are distinct molecules, in accordance with the localisation results. Overall, the resuits provide evidence for

Who reads Molecular differentiation in pea powdery-mildew haustoria: Identification of a 62-kDa N-linked glycoprotein unique to the haustorial plasma membrane?

Author

Andrew J. Mackie; Angela M. Roberts; James A. Callow; Jonathan R. Green

Publisher

Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0032-0935)

Published

1991

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)