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In situ Nuclear Matrix preparation in Drosophila melanogaster enabling genetic analysis of the nuclear architecture by Rashmi U. Pathak; Ashish Bihani; Rahul Sureka; Rakesh K. Mishra is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is In situ Nuclear Matrix preparation in Drosophila melanogaster enabling genetic analysis of the nuclear architecture about?

## Summary Nuclear Matrix (NuMat) is a biochemically defined entity that provides us with a snapshot of the features of the nuclear architecture. Here, we present a protocol to isolate and visualize NuMat __in situ__ in the intact embryo or tissues of __Drosophila melanogaster__ and its applications. We remove the chromatin to reveal underlying nuclear architectural components in organismal context. This protocol couples the power of __Drosophila__ genetics with cell biological observation of the nuclear architecture. For complete details on the use and execution of this protocol, please refer to Pathak et al. (2022), Sureka et al. (2018), and Pathak et al. (2013).

Who reads In situ Nuclear Matrix preparation in Drosophila melanogaster enabling genetic analysis of the nuclear architecture?

Author

Rashmi U. Pathak; Ashish Bihani; Rahul Sureka; Rakesh K. Mishra

Publisher

Elsevier BV

Published

2022

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)