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Can I read CDR1as regulates α-synuclein-mediated ischemic brain damage by controlling miR-7 availability on EtoBox?
CDR1as regulates α-synuclein-mediated ischemic brain damage by controlling miR-7 availability by Suresh L. Mehta; Anil K. Chokkalla; Saivenkateshkomal Bathula; Vijay Arruri; Bharath Chelluboina; Raghu Vemuganti is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is CDR1as regulates α-synuclein-mediated ischemic brain damage by controlling miR-7 availability about?
Transient focal ischemia decreased microRNA-7 (miR-7) levels, leading to derepression of its major target α-synuclein (α-Syn) that promotes secondary brain damage. Circular RNA CDR1as is known to regulate miR-7 abundance and function. Hence, we currently evaluated its functional significance after focal ischemia. Transient middle cerebral artery occlusion (MCAO) in adult mice significantly downregulated both CDR1as and miR-7 levels in the peri-infarct cortex between 3 and 72 h of reperfusion. Interestingly, neither pri-miR-7a nor 7b was altered in the ischemic brain. Intracerebral injection of an AAV9 vector containing a CDR1as gene significantly increased CDR1as levels by 21 days that persisted up to 4 months without inducing any observable toxicity in both sham and MCAO groups. Following transient MCAO, there was a significant increase in miR-7 levels and CDR1as binding to Ago2/miR-7 in the peri-infarct cortex of AAV9-CDR1as cohort compared with AAV9-Control cohort at 1 day of reperfusion. CDR1as overexpression significantly suppressed post-stroke α-Syn protein induction, promoted motor function recovery, decreased infarct size, and curtailed the markers of apoptosis, autophagy m
Who reads CDR1as regulates α-synuclein-mediated ischemic brain damage by controlling miR-7 availability?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Suresh L. Mehta; Anil K. Chokkalla; Saivenkateshkomal Bathula; Vijay Arruri; Bharath Chelluboina; Raghu Vemuganti
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)