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The Transition of Microglia to a Ramified Phenotype Is Associated with the Formation of Stable Acetylated and Detyrosinated Microtubules by Susanne Ilschner; Roland Brandt is a Neuroscience article available to read on EtoBox.
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In situ and in vitro, microglia can have different morphologies, which are thought to reflect distinct physiological activities. Two extreme forms are ameboid and ramified microglia. To study cytoskeletal changes during differentiation, we used defined cell culture systems to yield cultures of ameboid or ramified microglia from mouse brain. With respect to proliferation, secretion, receptor-expression, and phagocytosis, ramified microglia was generally less active. We found that the transition to a ramified phenotype was accompanied by an increase in the relative amount of acetylated and detyrosinated tubulin. Whereas the modified microtubules were restricted to regions close to the microtubule-organizing centers (MTOCs) in ameboid cells, acetylated microtubules were abundant in ramified cells, where they appeared to traverse from one process to another with no apparent anchoring at MTOCs. The increase in acetylated and detyrosinated microtubules was paralleled by an increased stability against nocodazoleinduced microtubule disassembly and by a lower rate of change in the length of the processes. Staining of retinal wholemounts confirmed the presence of acetylated microtubules in r
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It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Susanne Ilschner; Roland Brandt
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0894-1491)
- Published
- 1996
- Language
- EN
- Field
- Neuroscience (Life Sciences)