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Simultaneous Determination of First‐line Anti‐tuberculosis Drugs and Their Major Metabolic Ratios by Liquid Chromatography/tandem Mass Spectrometry by Sang Hoon Song; Sun Hee Jun; Kyoung Un Park; Yeomin Yoon; Jae Ho Lee; Jin Q Kim; Junghan Song is a Chemistry article available to read on EtoBox.
What is Simultaneous Determination of First‐line Anti‐tuberculosis Drugs and Their Major Metabolic Ratios by Liquid Chromatography/tandem Mass Spectrometry about?
## Abstract Monitoring of anti‐tuberculosis drug concentrations and dose adjustment can be helpful in cases that show poor response to treatment. Here, we describe a method that can rapidly and simultaneously measure the blood concentrations of four anti‐tuberculosis drugs (isoniazid, rifampicin, pyrazinamide, and ethambutol) and two major metabolic ratios (acetylisoniazid/isoniazid and 25‐desacetylrifampicin/rifampicin) using high‐performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS). A C18 reversed‐phase column and gradients of methanol in 0.3% formic acid and water were used for HPLC separation. The drug concentrations were determined by multiple reaction monitoring in positive ion mode and the assay performance was evaluated. We determined peak concentration ranges for each drug and acetylisoniazid/isoniazid and 25‐desacetylrifampicin/rifampicin ratios by analyzing 2‐h post‐dose samples in patients treated with standard dosing as a first‐line treatment. The preparation of 20 samples including two steps of deproteinization with 50% and 100% methanol was performed within 20 min and chromatographic separation was achieved within 4 min/sample. Interassay calibrati
Who reads Simultaneous Determination of First‐line Anti‐tuberculosis Drugs and Their Major Metabolic Ratios by Liquid Chromatography/tandem Mass Spectrometry?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Sang Hoon Song; Sun Hee Jun; Kyoung Un Park; Yeomin Yoon; Jae Ho Lee; Jin Q Kim; Junghan Song
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)
- Published
- 2007
- Language
- EN
- Field
- Chemistry (Physical Sciences)