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An Origami-based Colorimetric Sensor for Detection of Hydrogen Peroxide and Glucose Using Sericin Capped Silver Nanoparticles by Younes Mirzaei; Ali Gholami; Azarmidokht Sheini; Mohammad Mahdi Bordbar is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is An Origami-based Colorimetric Sensor for Detection of Hydrogen Peroxide and Glucose Using Sericin Capped Silver Nanoparticles about?

The hydrogen peroxide (H~2~O~2~) measurement is considered highly important in industrial wastewater quality assessment, environmental protection, and disease detection. Here, a simple high-performance paper-based sensor is proposed for rapid and in situ detection of H~2~O~2~. To this end, 3,3′,5,5′-tetramethylbenzidine is embedded in the sensor to act as a color indicator, whose reaction with hydrogen peroxide is catalyzed by a silver nanozyme modified by sericin. The result of the reaction clarified by the appearance of blue color in the sensor detection zone is received by a portable scanner, while also calculating its intensity by image analysis software. This method is sensitive to hydrogen peroxide in the concentration range of 0.5‒240 mg/dL, providing a detection limit of 0.15 mg/dL. The ability of the sensor to determine glucose is also evaluated by adding a layer containing glucose oxidase enzyme to the sensor structure. A desirable response is obtained in the range of 1.0‒160 mg/dL, together with a detection limit of 0.37 mg/dL. Accordingly, the proposed sensor shows satisfactory results compared to clinical methods for monitoring the amount of glucose in biological sampl

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Author

Younes Mirzaei; Ali Gholami; Azarmidokht Sheini; Mohammad Mahdi Bordbar

Publisher

Springer Science and Business Media LLC

Published

2023

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)