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Analysis of Multi-lineage Gene Expression Dynamics During Primordial Germ Cell Induction from Human Induced Pluripotent Stem Cells by Fang, Fang; Li, Zili; Zhao, Qian; Xiong, Chengliang; Ni, Ke is a Medicine article available to read on EtoBox.

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## Background In mammals, specification of primordial germ cells (PGCs) is established in the early post­implantation embryo. The bone morphogenetic protein (BMP)-SMAD and WNT3-β-catenin signaling initiate the gene regulatory network for PGC specification. The activation of SOX17-BLIMP1 axis is critical for human PGC program. Moreover, EpCAM and INTEGRINα6 were identified as surface markers of human PGC-like cells (PGCLCs) recently. However, the signaling mechanism for PGC specification in non­rodent mammals remains to be clarified. ## Methods We differentiated human induced pluripotent stem cells (hiPSCs) into PGCLCs in vitro in response to Activin A and BMP4. The percentage of EpCAM/INTEGRINα6 double-positive cells (PGCLCs) was analyzed by flow cytometry. The expression of PGC genes was evaluated by qRT-PCR and immunofluorescence. The expression dynamic of multi-lineage genes during the differentiation process was evaluated by qRT-PCR. ## Results Under the stimulation for PGCLC induction, the embryoids derived from hiPSCs initiated significant upregulation of the early PGC genes (BLIMP1, TFAP2C, and NANOS3), but maintained low or no levels of DPPA3 and late PGC genes (DAZL and DD

Who reads Analysis of Multi-lineage Gene Expression Dynamics During Primordial Germ Cell Induction from Human Induced Pluripotent Stem Cells?

Author

Fang, Fang; Li, Zili; Zhao, Qian; Xiong, Chengliang; Ni, Ke

Publisher

BioMed Central; Springer (Biomed Central Ltd.); Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1757-6512)

Published

2020

Language

EN

Field

Medicine (Health Sciences)