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Can I read The Characteristics and Stabilization of a Caffeine Demethylase Enzyme Complex on EtoBox?
The Characteristics and Stabilization of a Caffeine Demethylase Enzyme Complex by Odafe F. P. Sideso; Allison C. Marvier; Nikolaos A. Katerelos; Peter W. Goodenough is a Agricultural and Biological Sciences article available to read on EtoBox.
What is The Characteristics and Stabilization of a Caffeine Demethylase Enzyme Complex about?
Cell‐free extracts (CFE) from __Pseudomonas putida__, cultured on caffeine as sole carbon and/or nitrogen source, contained proteins which demethylate caffeine. Caffeine metabolism correlated with production of two major proteins (43.5 and 36.6 kDa); we suggest that one or both of these are caffeine demethylase enzymes. HPLC analysis of caffeine degradation by __P. putida__ showed that caffeine is converted to uric acid via 3–7‐dimethylxanthine, 7‐methylxanthine and xanthine. The stability of caffeine demethylase was greatly improved by the use of cryoprotectants and freeze drying to low moisture contents. Freeze‐dried cell‐free extracts maintained 83% of activity after 200 days of storage at 4 °C.
Who reads The Characteristics and Stabilization of a Caffeine Demethylase Enzyme Complex?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Odafe F. P. Sideso; Allison C. Marvier; Nikolaos A. Katerelos; Peter W. Goodenough
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-5423)
- Published
- 2001
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)