Can I read Embryonic Ethanol Exposure Alters Expression of Sox2 and Other Early Transcripts in Zebrafish, Producing Gastrulation Defects on EtoBox?

Embryonic Ethanol Exposure Alters Expression of Sox2 and Other Early Transcripts in Zebrafish, Producing Gastrulation Defects by Sarmah, Swapnalee; Srivastava, Rajneesh; McClintick, Jeanette N.; Janga, Sarath C.; Edenberg, Howard J.; Marrs, James A. is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Embryonic Ethanol Exposure Alters Expression of Sox2 and Other Early Transcripts in Zebrafish, Producing Gastrulation Defects about?

Ethanol exposure during prenatal development causes fetal alcohol spectrum disorder (FASD), the most frequent preventable birth defect and neurodevelopmental disability syndrome. The molecular targets of ethanol toxicity during development are poorly understood. Developmental stages surrounding gastrulation are very sensitive to ethanol exposure. To understand the effects of ethanol on early transcripts during embryogenesis, we treated zebrafish embryos with ethanol during pre-gastrulation period and examined the transcripts by Affymetrix GeneChip microarray before gastrulation. We identified 521 significantly dysregulated genes, including 61 transcription factors in ethanol-exposed embryos. Sox2, the key regulator of pluripotency and early development was significantly reduced. Functional annotation analysis showed enrichment in transcription regulation, embryonic axes patterning, and signaling pathways, including Wnt, Notch and retinoic acid. We identified all potential genomic targets of 25 dysregulated transcription factors and compared their interactions with the ethanol-dysregulated genes. This analysis predicted that Sox2 targeted a large number of ethanol-dysregulated genes

Who reads Embryonic Ethanol Exposure Alters Expression of Sox2 and Other Early Transcripts in Zebrafish, Producing Gastrulation Defects?

Author

Sarmah, Swapnalee; Srivastava, Rajneesh; McClintick, Jeanette N.; Janga, Sarath C.; Edenberg, Howard J.; Marrs, James A.

Publisher

Springer Science and Business Media LLC; London: Nature Publishing Group; [London]: Springer Nature; Nature Publishing Group; Society for Mining, Metallurgy and Exploration Inc.; Research Square; Springer Nature; Island Press (ISSN 2045-2322)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)