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This document describes an experiment for separating a mixture of methylene blue and fluorescein using adsorption chromatography with an acidic alumina column as the stationary phase. Methylene blue was eluted first due to its positive charge, while fluorescein, with negative charges, was adsorbed more strongly to the alumina and was eluted later when the eluent was changed to one with a higher pH. The obtained elution profile showed...

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