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The effect of local delivery of PDGF-BB on attachment of human periodontal ligament fibroblasts to periodontitis-affected root surfaces – in vitro by Ahmed Y. Gamal; Jason M. Mailhot is a Dentistry article available to read on EtoBox.

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## Abstract **Background/aims:** The purpose of this study was to determine at what concentration does platelet‐derived growth factor‐BB (PDGF‐BB) provide for optimal stimulation of human periodontal ligament fibroblasts (PDL) to adhere to periodontitis affected root surfaces. **Method:** 80 root dentine specimens were prepared from extracted periodontally diseased teeth obtained from patients ranging in age between 35 to 60 years. The root dentine specimens were associated with the subgingival area opposing the periodontal pocket for each extracted tooth. 10 healthy root dentine specimens were obtained from teeth extracted for orthodontic reasons and served as controls. The specimens were distributed into 9 groups (10 specimens in each). In group 1, PDL fibroblasts were cultured on the specimen surface of a diseased treated control. In group 2, PDL fibroblasts were cultured on the specimen surface of a healthy control. In groups 3 to 9, PDL fibroblasts were cultured on a pretreated specimen surface with concentrations ranging from 5, 10, 20, 50, 100, 200 and 300 ng/ml PDGF‐BB, respectively. After 24 h incubation, the media were removed, specimens were fixed, processed for SEM vie

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Author

Ahmed Y. Gamal; Jason M. Mailhot

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0303-6979)

Published

2000

Language

EN

Field

Dentistry (Health Sciences)