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Can I read Development of a Two-stage Limb Ischemia Model to Better Simulate Human Peripheral Artery Disease on EtoBox?
Development of a Two-stage Limb Ischemia Model to Better Simulate Human Peripheral Artery Disease by Krishna, Smriti M.; Omer, Safraz Mohamed; Li, Jiaze; Morton, Susan K.; Jose, Roby J.; Golledge, Jonathan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Development of a Two-stage Limb Ischemia Model to Better Simulate Human Peripheral Artery Disease about?
Peripheral arterial disease (PAD) develops due to the narrowing or blockage of arteries supplying blood to the lower limbs. Surgical and endovascular interventions are the main treatments for advanced PAD but alternative and adjunctive medical therapies are needed. Currently the main preclinical experimental model employed in PAD research is based on induction of acute hind limb ischemia (HLI) by a 1-stage procedure. Since there are concerns regarding the ability to translate findings from this animal model to patients, we aimed to develop a novel clinically relevant animal model of PAD. HLI was induced in male Apolipoprotein E (__ApoE__^__−/−__^) deficient mice by a 2-stage procedure of initial gradual femoral artery occlusion by ameroid constrictors for 14 days and subsequent excision of the femoral artery. This 2-stage HLI model was compared to the classical 1-stage HLI model and sham controls. Ischemia severity was assessed using Laser Doppler Perfusion Imaging (LDPI). Ambulatory ability was assessed using an open field test, a treadmill test and using established scoring scales. Molecular markers of angiogenesis and shear stress were assessed within gastrocnemius muscle tissue
Who reads Development of a Two-stage Limb Ischemia Model to Better Simulate Human Peripheral Artery Disease?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Krishna, Smriti M.; Omer, Safraz Mohamed; Li, Jiaze; Morton, Susan K.; Jose, Roby J.; Golledge, Jonathan
- Publisher
- Springer Science and Business Media LLC; London: Nature Publishing Group; [London]: Springer Nature; Nature Publishing Group; Society for Mining, Metallurgy and Exploration Inc.; Research Square; Springer Nature; Island Press (ISSN 2045-2322)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)