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Pentoxifylline sensitizes human cervical tumor cells to cisplatin-induced apoptosis by suppressing NF-kappa B and decreased cell senescence by Georgina Hernandez-Flores; Pablo C Ortiz-Lazareno; Jose Manuel Lerma-Diaz; Jorge R Dominguez-Rodriguez; Luis F Jave-Suarez; Adriana del C Aguilar-Lemarroy; Ruth de Celis-Carrillo; Susana del Toro-Arreola; Yessica C Castellanos-Esparza; Alejandro Bravo-Cuellar is a Medicine article available to read on EtoBox.

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## Background Worldwide, cervical cancer is the second most common causes of cancer in women and represents an important mortality rate. Cisplatin (CIS) is a very important antitumoral agent and can lead tumor cells toward two important cellular states: apoptosis and senescence. In some types of cancers pentoxifylline (PTX) sensitizes these cells to the toxic action of chemotherapeutics drugs such as adriamycin, inducing apoptosis. In the present work, we studied __in vitro__ whether PTX alone or in combination with CIS induces apoptosis and/or senescence in cervix cancer HeLa and SiHa cell lines infected with HPV types 16 and 18, respectively, as well as in immortalized keratinocytyes HaCaT cells. ## Methods HeLa (HPV 18+), SiHa (HPV 16+) cervix cancer cells and non-tumorigenic immortalized HaCaT cells (control) were treated with PTX, CIS or both. The cellular toxicity and survival fraction of PTX and CIS were determinate by WST-1 and clonogenic assays respectively. Apoptosis, caspase activation and phosphorylation of ERK1/2, p38, p65 (NF-κB), Bcl-2 and Bcl-XL anti-apoptotic proteins were determinated by flow cytometry. Senescence by microscopy. Phosphorylation of IκBα and IκB tot

Who reads Pentoxifylline sensitizes human cervical tumor cells to cisplatin-induced apoptosis by suppressing NF-kappa B and decreased cell senescence?

Author

Georgina Hernandez-Flores; Pablo C Ortiz-Lazareno; Jose Manuel Lerma-Diaz; Jorge R Dominguez-Rodriguez; Luis F Jave-Suarez; Adriana del C Aguilar-Lemarroy; Ruth de Celis-Carrillo; Susana del Toro-Arreola; Yessica C Castellanos-Esparza; Alejandro Bravo-Cuellar

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2001]-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1471-2407)

Published

2011

Language

EN

ISBN

9781471240713

Field

Medicine (Health Sciences)