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Melanophore pigment dispersion responses to agonists show two patterns of sensitivity to inhibitors of cAMP-dependent protein kinase and protein kinase C by Timothy S. McClintock; Joshua P. Rising; Michael R. Lerner is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Melanophore pigment dispersion responses to agonists show two patterns of sensitivity to inhibitors of cAMP-dependent protein kinase and protein kinase C about?

Melanophore pigment dispersion is a sensitive bioassay for activation of the adenylyl cyclase and phospholipase C second-messenger pathways. The necessity of protein kinase activation in causing pigment dispersion was confirmed for eight agonists of endogenous melanophore receptors and for two transfected receptors. All agonists and receptors previously shown to elevate intracellular cAMP in melanophores-melanocyte stimulating hormone, light, (-1 norepinephrine, 5-hydroxytrptamine, and the P2-adrenergic receptor-were able to stimulate pigment dispersion in the presence of Ro31-8220, a potent inhibitor of protein kinase C, but were blocked in the presence of H89, an inhibitor of CAMPdependent protein kinase. The bombesin receptor, which elevates intracellular IP, in melanophores, was unable to stimulate pigment dispersion in the presence of Ro31-8220 or H89. Agonists whose mechanism of activation of pigment dispersion are unknown were also tested. Endothelin 3 responses were blocked by both H89 and Ro31-8220, predicting coupling to phospholipase C. Vasoactive intestinal polypeptide, oxytocin, and calcitonin gene-related peptide P responses were blocked only by H89, predicting coupli

Who reads Melanophore pigment dispersion responses to agonists show two patterns of sensitivity to inhibitors of cAMP-dependent protein kinase and protein kinase C?

Author

Timothy S. McClintock; Joshua P. Rising; Michael R. Lerner

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1996

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)