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Noncanonical Amino Acids as Doubly Bio-orthogonal Handles for One-pot Preparation of Protein Multiconjugates by Yong Wang; Jingming Zhang; Boyang Han; Linzhi Tan; Wenkang Cai; Yuxuan Li; Yeyu Su; Yutong Yu; Xin Wang; Xiaojiang Duan; Haoyu Wang; Xiaomeng Shi; Jing Wang; Xing Yang; Tao Liu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Noncanonical Amino Acids as Doubly Bio-orthogonal Handles for One-pot Preparation of Protein Multiconjugates about?
## Abstract Genetic encoding of noncanonical amino acid (ncAA) for site-specific protein modification has been widely applied for many biological and therapeutic applications. To efficiently prepare homogeneous protein multiconjugates, we design two encodable noncanonical amino acids (ncAAs), 4-(6-(3-azidopropyl)-s-tetrazin-3-yl) phenylalanine (pTAF) and 3-(6-(3-azidopropyl)-s-tetrazin-3-yl) phenylalanine (mTAF), containing mutually orthogonal and bioorthogonal azide and tetrazine reaction handles. Recombinant proteins and antibody fragments containing the TAFs can easily be functionalized in one-pot reactions with combinations of commercially available fluorophores, radioisotopes, PEGs, and drugs in a plug-and-play manner to afford protein dual conjugates to assess combinations of tumor diagnosis, image-guided surgery, and targeted therapy in mouse models. Furthermore, we demonstrate that simultaneously incorporating mTAF and a ketone-containing ncAA into one protein via two non-sense codons allows preparation of a site-specific protein triconjugate. Our results demonstrate that TAFs are doubly bio-orthogonal handles for efficient and scalable preparation of homogeneous protein mu
Who reads Noncanonical Amino Acids as Doubly Bio-orthogonal Handles for One-pot Preparation of Protein Multiconjugates?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Yong Wang; Jingming Zhang; Boyang Han; Linzhi Tan; Wenkang Cai; Yuxuan Li; Yeyu Su; Yutong Yu; Xin Wang; Xiaojiang Duan; Haoyu Wang; Xiaomeng Shi; Jing Wang; Xing Yang; Tao Liu
- Publisher
- Springer Science and Business Media LLC
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)