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Hyris bCUBE SARS-CoV-2 rapid molecular saliva testing: a POCT innovation on its way by Andrea Padoan; Chiara Cosma; Ada Aita; Filippo Navaglia; Daniela Basso; Gianfranco Giannella; Mario Plebani is a Medicine article available to read on EtoBox.

What is Hyris bCUBE SARS-CoV-2 rapid molecular saliva testing: a POCT innovation on its way about?

## Abstract ## Objectives The reliable identification of individuals with SARS-CoV-2 infection is the cornerstone for containing viral spread. Rapid molecular point-of-care testing (POCT) of saliva might reduce analysis time, thus increasing the efficacy of contact tracing. In this study, a new POCT RT-PCR assay for the detection of SARS-CoV-2 RNA in saliva was evaluated and compared with an already validated CE-IVD method. ## Methods An evaluation was made of 160 left-over salivary samples (27 frozen, kept at −80 °C and 133 fresh), collected using Salivette (Sarstedt, Germany). Samples were analyzed by TaqPath COVID-19 CE-IVD RT-PCR kit, QuantStudio5 Real-Time (Applied Biosystems, USA) (TaqPath) and bKIT Virus Finder COVID-19 Saliva (Hyris Global Diagnostics, Italy). Performances of three- and fivefold pooling strategies were also evaluated. Blood assay interference in saliva was also tested with Hyris. ## Results On using TaqPath, SARS-CoV-2 positivity was detected in 35 samples. Another 10 positive samples were artificially-generated by blind mixing of positive with negative samples. Hyris positive and negative percentages of agreement were 97.6 (95% CI: 87.2–99.9%) and 100 (95%

Who reads Hyris bCUBE SARS-CoV-2 rapid molecular saliva testing: a POCT innovation on its way?

Author

Andrea Padoan; Chiara Cosma; Ada Aita; Filippo Navaglia; Daniela Basso; Gianfranco Giannella; Mario Plebani

Publisher

Walter de Gruyter GmbH

Published

2022

Language

EN

Field

Medicine (Health Sciences)