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Overexpression of Insig-1 protects β cell against glucolipotoxicity via SREBP-1c by Ke Chen; ping jin; Hong-hui He; Yan-hong Xie; Xiao-yun Xie; Zhao-hui Mo is a Medicine article available to read on EtoBox.

What is Overexpression of Insig-1 protects β cell against glucolipotoxicity via SREBP-1c about?

## Background High glucose induced lipid synthesis leads to β cell glucolipotoxicity. Sterol regulatory element binding protein-1c (SREBP-1c) is reported to be partially involved in this process. Insulin induced gene-1 (Insig-1) is an important upstream regulator of Insig-1-SREBPs cleavage activating protein (SCAP)-SREBP-1c pathway. Insig-1 effectively blocks the transcription of SREBP-1c, preventing the activation of the genes for lipid biosynthesis. In this study, we aimed to investigate whether Insig-1 protects β cells against glucolipotoxicity. ## Methods An Insig-1 stable cell line was generated by overexpression of Insig-1 in INS-1 cells. The expression of Insig-1 was evaluated by RT-PCR and Western blotting, then, cells were then treated with standard (11.2 mM) or high (25.0 mM) glucose for 0 h, 24 h and 72 h. Cell viability, apoptosis, glucose stimulated insulin secretion (GSIS), lipid metabolism and mRNA expression of insulin secretion relevant genes such as IRS-2, PDX-1, GLUT-2, Insulin and UCP-2 were evaluated. ## Results We found that Insig-1 suppressed the high glucose induced SREBP-1c mRNA and protein expression. Our results also showed that Insig-1 overexpression pro

Who reads Overexpression of Insig-1 protects β cell against glucolipotoxicity via SREBP-1c?

Author

Ke Chen; ping jin; Hong-hui He; Yan-hong Xie; Xiao-yun Xie; Zhao-hui Mo

Publisher

BioMed Central; Springer (Biomed Central Ltd.); BioMed Central Ltd.; [Basel Switzerland]: Karger; London: 2009-; Springer Science and Business Media LLC; S. Karger AG; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1021-7770)

Published

2011

Language

EN

Field

Medicine (Life Sciences)