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P2X7 receptor-dependent ATP-induced shedding of CD27 in mouse lymphocytes by Hana Moon; Hye-Young Na; Kyung Hee Chong; Tae Jin Kim is a Immunology and Microbiology article available to read on EtoBox.

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The ATP-gated P2X 7 cell surface receptor belongs to the P2X purinoreceptor family, and is expressed abundantly by immune cells. This receptor is involved in several inflammatory and immunological processes, including the secretion of IL-1 by activated macrophages. Here, we demonstrate that CD27, a cell surface molecule, which is involved in the interaction between immune cells and implicated in the immunological memory of the T and B cells, is released rapidly upon treatment with low ATP concentrations, via the activation of the P2X 7 receptor. The surface expression of CD27 on the T and B cells has been shown to be down-regulated as the result of ATP treatment, and the soluble form of CD27 was detected readily in the supernatants of splenocytes, which were cultured along with ATP treatment. The shedding of CD27 was blocked by KN-62, a chemical P2X 7 inhibitor, and was also efficiently triggered by treatment with 2,3-O-(4-benzoyl-benzoyl)-ATP. The shedding of CD27 from mouse splenic T cells was observed to occur within 5 min of treatment with 300 M ATP, whereas treatment with PMA or ionomycin was not determined to trigger the shedding of CD27 until treatment had been applied for a

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Author

Hana Moon; Hye-Young Na; Kyung Hee Chong; Tae Jin Kim

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0165-2478)

Published

2006

Language

EN

Field

Immunology and Microbiology (Life Sciences)