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Factors affecting ferulic acid release from Brewer’s spent grain by Fusarium oxysporum enzymatic system by Charilaos Xiros; Maria Moukouli; Evangelos Topakas; Paul Christakopoulos is a Engineering article available to read on EtoBox.

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In this study, the factors affecting ferulic acid (FA) release from Brewer's spent grain (BSG), by the crude enzyme extract of Fusarium oxysporum were investigated. In order to evaluate the importance of the multienzyme preparation on FA release, the synergistic action of feruloyl esterase (FAE, FoFaeC-12213) and xylanase (Trichoderma longibrachiatum M3) monoenzymes was studied. More than double amount of FA release (1 mg g À1 dry BSG) was observed during hydrolytic reactions by the crude enzyme extract compared to hydrolysis by the monoenzymes (0.37 mg g À1 dry BSG). The protease content of the crude extract and the inhibitory effect of FA as an end-product were also evaluated concerning their effect on FA release. The protease treatment prior to hydrolysis by monoenzymes enhanced FA release about 100%, while, for the first time in literature, FA in solution found to have a significant inhibitory effect on FAE activity and on total FA release.

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Author

Charilaos Xiros; Maria Moukouli; Evangelos Topakas; Paul Christakopoulos

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0960-8524)

Published

2009

Language

EN

Field

Engineering (Physical Sciences)