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Can I read Elevated Expression of Vacuolar Proton Pump Genes and Cellular Ph in Cisplatin Resistance on EtoBox?
Elevated Expression of Vacuolar Proton Pump Genes and Cellular Ph in Cisplatin Resistance by Tadashi Murakami; Izumi Shibuya; Tomoko Ise; Zhe-Sheng Chen; Shin-ichi Akiyama; Masayuki Nakagawa; Hiroto Izumi; Toshitaka Nakamura; Ken-ichi Matsuo; Yuji Yamada; Kimitoshi Kohno is a Medicine article available to read on EtoBox.
What is Elevated Expression of Vacuolar Proton Pump Genes and Cellular Ph in Cisplatin Resistance about?
V-ATPases are proton-translocating enzymes, which are found not only in numerous intracellular organelles but also in the plasma membranes of many eukaryotic cells. Using differential display, we have identified one of the proton pump subunit genes, ATP6C, as a cisplatin-inducible gene. Northern blot analysis demonstrated that expression of other members of the subunit is inducible by cisplatin treatment. Proton pump gene expression is also upregulated in 3 independent cisplatin-resistant cell lines but not in vincristine-or etoposide-resistant cell lines. Cellular pH was significantly higher in cisplatin-resistant cells than in sensitive parental cells. In vitro DNA-binding activity of cisplatin was markedly increased in acidic conditions, suggesting that the cytotoxicity of cisplatin is modulated by cellular pH. Furthermore, the proton pump inhibitor bafilomycin can synergistically potentiate the cytotoxicity of cisplatin but not of etoposide or camptothecin. These results indicate that cellular pH is one of the critical parameters for effective cancer chemotherapy with cisplatin.
Who reads Elevated Expression of Vacuolar Proton Pump Genes and Cellular Ph in Cisplatin Resistance?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Tadashi Murakami; Izumi Shibuya; Tomoko Ise; Zhe-Sheng Chen; Shin-ichi Akiyama; Masayuki Nakagawa; Hiroto Izumi; Toshitaka Nakamura; Ken-ichi Matsuo; Yuji Yamada; Kimitoshi Kohno
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 2001
- Language
- EN
- Field
- Medicine (Health Sciences)