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Can I read In Vitro Effect of the Cell-Free Supernatant of the Lactobacillus casei Strain IMAU60214 against the Different Pathogenic Properties of Diarrheagenic Escherichia coli on EtoBox?

In Vitro Effect of the Cell-Free Supernatant of the Lactobacillus casei Strain IMAU60214 against the Different Pathogenic Properties of Diarrheagenic Escherichia coli by Luz María Rocha-Ramírez; Ulises Hernández-Chiñas; Silvia Selene Moreno-Guerrero; Arturo Ramírez-Pacheco; Carlos A. Eslava is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is In Vitro Effect of the Cell-Free Supernatant of the Lactobacillus casei Strain IMAU60214 against the Different Pathogenic Properties of Diarrheagenic Escherichia coli about?

Enteroaggregative __Escherichia coli__ (EAEC) and enterohemorrhagic __E. coli__ (EHEC) are __E. coli__ pathotypes associated with unmanageable diarrhea in children and adults. An alternative to the treatment of infections caused by these microorganisms is the use of the bacteria of the __Lactobacillus__ genus; however, the beneficial effects on the intestinal mucosa are specific to the strain and species. The interest of this study consisted of analyzing the coaggregation properties of __Lactobacillus casei__ IMAU60214, as well as the effect of cell-free supernatant (CSF) on growth and anti-cytotoxic activity in a cell model of the human intestinal epithelium for an agar diffusion assay (HT-29) and the inhibition of biofilm formation on plates of DEC strains of the EAEC and EHEC pathotypes. The results showed that __L. casei__ IMAU60214 exhibits time-dependent coaggregation (35–40%) against EAEC and EHEC that is similar to the control __E. coli__ ATCC 25922. The CSF showed antimicrobial activity (20–80%) against EAEC and EHEC depending on the concentration. In addition, the formation and dispersion of biofilms of the same strains decrease, and the proteolytic pre-treatment with cat

Who reads In Vitro Effect of the Cell-Free Supernatant of the Lactobacillus casei Strain IMAU60214 against the Different Pathogenic Properties of Diarrheagenic Escherichia coli?

It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.

Author
Luz María Rocha-Ramírez; Ulises Hernández-Chiñas; Silvia Selene Moreno-Guerrero; Arturo Ramírez-Pacheco; Carlos A. Eslava
Publisher
MDPI AG
Published
2023
Language
EN
Field
Biochemistry, Genetics and Molecular Biology (Life Sciences)

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