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This study developed species-specific multiplex real-time PCR assays to trace small pelagic fishes in the North Pacific using environmental DNA (eDNA). The assays were validated with eDNA samples collected from fishing nets, revealing seasonal patterns in fish presence and correlating eDNA quantities with fish biomass. The methods aim to enhance understanding of the ecological behavior and migration routes of these important fish species, contributing to sustainable fisheries management.

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kwk42l100

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EN