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Background: T follicular helper (Tfh) cells are a T helper subtype specialized in supporting B cell activation, resulting in B cell mediated humoral immunity. In transplant patients, this can lead to antibody mediated rejection. Tacrolimus (TAC), and sirolimus (SRL) are two commonly used immunosuppressive drugs used in transplantation; however, their effect on Tfh generation is largely unknown. In this study we therefore studied in vitro Tfh like cell formation under immunosuppressed conditions. Methods: Isolated naive T cells were polarized to Tfh-like cells in the presence of different concentrations of TAC or SRL, (ranging from 0.5 to 20 ng/ ml and from 1 to 10 ng/ml, respectively). Tfh-like cells were defined as CD4 + CXCR5 + T cells, which also express PD-1 and ICOS. To demonstrate their functionality, we co-cultured these cells with isolated B cells in the presence of alloantigen and then analyzed the Tfh-like cells and their ability to help B cells. Results: We found that both TAC and SRL significantly inhibited the differentiation into Tfh-like cells. Therapeutic concentrations of TAC and SRL reduced the percentage of PD-1+ and ICOS+ Tfh cells compared to controls without i

Who reads Moderated Poster?

Publisher

Frontiers Media SA

Published

2019

Language

EN

Field

Medicine (Health Sciences)