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Differential distribution of release‐related proteins in the hippocampal CA3 area as revealed by freeze‐fracture replica labeling by Akari Hagiwara; Yugo Fukazawa; Maki Deguchi‐Tawarada; Toshihisa Ohtsuka; Ryuichi Shigemoto is a Neuroscience article available to read on EtoBox.
What is Differential distribution of release‐related proteins in the hippocampal CA3 area as revealed by freeze‐fracture replica labeling about?
## Abstract Synaptic vesicle release occurs at a specialized membrane domain known as the presynaptic active zone (AZ). Several membrane proteins are involved in the vesicle release processes such as docking, priming, and exocytotic fusion. Cytomatrix at the active zone (CAZ) proteins are structural components of the AZ and are highly concentrated in it. Localization of other release‐related proteins including target soluble N‐ethylmaleimide‐sensitive‐factor attachment protein receptor (t‐SNARE) proteins, however, has not been well demonstrated in the AZ. Here, we used sodium dodecyl sulfate‐digested freeze‐fracture replica labeling (SDS‐FRL) to analyze quantitatively the distribution of CAZ and t‐SNARE proteins in the hippocampal CA3 area. The AZ in replicated membrane was identified by immunolabeling for CAZ proteins (CAZ‐associated structural protein [CAST] and Bassoon). Clusters of immunogold particles for these proteins were found on the P‐face of presynaptic terminals of the mossy fiber and associational/commissural (A/C) fiber. Co‐labeling with CAST revealed distribution of the t‐SNARE proteins syntaxin and synaptosomal‐associated protein of 25 kDa (SNAP‐25) in the AZ as wel
Who reads Differential distribution of release‐related proteins in the hippocampal CA3 area as revealed by freeze‐fracture replica labeling?
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- Author
- Akari Hagiwara; Yugo Fukazawa; Maki Deguchi‐Tawarada; Toshihisa Ohtsuka; Ryuichi Shigemoto
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0021-9967)
- Published
- 2005
- Language
- EN
- Field
- Neuroscience (Life Sciences)