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On-chip Capillary Electrophoresis for Alkaline Phosphatase Testing by Yuji Murakami; Tomoyuki Morita; Takashi Kanekiyo; Eiichi Tamiya is a Engineering article available to read on EtoBox.

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We fabricated an on-chip capillary electrophoresis device for blood analysis. An on-chip capillary electrophoresis device was photolithographically fabricated on a glass chip. Alkaline phosphatase (ALP) was employed as a sample enzyme. Small amounts of enzyme in the mixture of other proteins were detected with the electrophoretically mediated microanalysis (EMMA) method. Fluorescein diphosphate was used as fluorogenic substrate. The detection of ALP activity was achieved with laser-induced fluorescence monitoring fluorescein that was produced in enzyme reaction in capillary. Several methods to reduce the adhesion of protein are also discussed.

Who reads On-chip Capillary Electrophoresis for Alkaline Phosphatase Testing?

Author

Yuji Murakami; Tomoyuki Morita; Takashi Kanekiyo; Eiichi Tamiya

Publisher

Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)

Published

2001

Language

EN

Field

Engineering (Physical Sciences)