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What is Caspase Detection in Apoptosis via Western Blot about?

Activated caspases are detected on a western blot as lighter bands representing each cleaved caspase dimer half. Apoptotic cells are detected by fluorescent labeling of cut DNA ends using the TUNEL assay, which involves adding fluorescent UTP to cut DNA ends using terminal deoxynucleotidyl transferase. Localization of fluorescent cytochrome C can also detect apoptosis at the single cell level by its redistribution from punctate mitochondrial labeling to a diffuse cytoplasmic pattern upon apoptosis induction

Author

g2gusc38

Language

EN