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Scaffold-free 3D cellulose acetate membrane-based cultures form large cartilaginous constructs by S. Mayer-Wagner; T. S. Schiergens; B. Sievers; J. I. Redeker; B. Schmitt; A. Buettner; V. Jansson; P. E. Müller is a Medicine article available to read on EtoBox.

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## Scaffold -free three-dimensional (3D) cultures provide clinical potential in cartilage regeneration. The purpose of this study was to characterize a scaffold-free 3D membrane-based culture system, in which human articular chondrocytes were cultivated on a cellulose acetate membrane filter, and compare it to pellet and monolayer cultures. Chondrocytes were expanded in monolayer culture for up to 5 passages, transferred to membrane-based or pellet cultures and harvested after 7 or 21 days. The chondrogenic potential was assessed by histology (toluidine blue, safranin-O), immunohistochemistry for collagen type II and quantitative analysis of collagen type II α 1 (COL2A1). Membrane-based cultures (P1) formed flexible disc-like constructs (diameter 4000 μm, thickness 150 μm) with a large smooth surface after 7 days. Positive safranin-O and collagen type II staining was found in membrane-based and pellet cultures at P1-3. Expression of COL2A1 after 7 days was increased in both culture systems compared to monolayer culture up to P3, whereas cells from monolayer >P3 did not redifferentiate. The best results for COL2A1 expression were obtained from membrane-based cultures at P1. After 21

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Author

S. Mayer-Wagner; T. S. Schiergens; B. Sievers; J. I. Redeker; B. Schmitt; A. Buettner; V. Jansson; P. E. Müller

Publisher

Hindawi Limited

Published

2011

Language

EN

Field

Medicine (Health Sciences)