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Release of WBC-derived IL-1 receptor antagonist into supernatants of RBCs: influence of storage time and filtration by Ivana Büttnerova; Hans Bäumler; Florian Kern; Hartmut Radtke; Hans-Dieter Volk; Holger Kiesewetter; Wolf-Dietrich Döcke is a Medicine article available to read on EtoBox.

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## Background: Transfusion-associated immunodepression may be related to the transfer of immunoinhibitory cytokines with blood components. ## Study design and methods: After evidence of increasing concentrations of il-1 receptor antagonist (il-1ra) but not of il-10 was obtained in supernatants of stored rbc units that were wbc-reduced by centrifugation (c-rbcs) in a pilot study, il-1ra concentrations were determined weekly in supernatants of c-rbcs and in units that underwent prestorage wbc reduction by in-line filtration (f-rbcs) over a 49-day storage. for assessing total il-1ra content, complete cell lysis by repeated freezing and thawing was done. the results were related to the changes in wbc count during storage. the dependency of il-1ra content on preparation procedures was assessed. ## Results: The prestorage il-1ra concentration in c-rbcs (859 +/- 218 pg/ml) was significantly higher than in f-rbc (75 +/- 13 pg/ml). whereas no changes were seen in f-rbcs during storage, il-1ra levels in c-rbc supernatants drastically increased to levels about 50 times those in normal plasma (16,327 +/- 2,686 pg/ml on day 49). follow-up analysis revealed stringent correlation between il-1ra r

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Author

Ivana Büttnerova; Hans Bäumler; Florian Kern; Hartmut Radtke; Hans-Dieter Volk; Holger Kiesewetter; Wolf-Dietrich Döcke

Publisher

Elsevier Science; John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Elsevier; Elsevier BV; Wiley (ISSN 0372-1248)

Published

2001

Language

EN

Field

Medicine (Health Sciences)