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Fiber Diameters Control Osteoblastic Cell Migration and Differentiation in Electrospun Gelatin by Kristin Sisson; Chu Zhang; Mary C. Farach-Carson; D. Bruce Chase; John F. Rabolt is a Engineering article available to read on EtoBox.

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## Abstract Defined electrospinning conditions were used to create scaffolds with different fiber diameters to investigate their interactions with osteoblastic MG63 cells. Nonwoven gelatin scaffolds were electrospun with varied fiber diameters to investigate the effect of fiber size and resultant porosity on cell proliferation, viability, migration, and differentiation. The low toxicity solvent acetic acid:ethyl acetate:water ratio and gelatin concentrations were optimized to create small and large diameter fibers. The fiber diameters obtained by this procedure were 110 ± 40 nm for the small and 600 ± 110 nm for the large fibers. Cell viability assays showed that MG63 cells grew similarly on both fibers at the early time point (day 3) but preferred the scaffold with large diameter fibers by the later time points (day 5 and day 7). Confocal microscopic imaging showed that MG63 cells migrated poorly (maximum depth of 18 μm) into the scaffold of small diameter fibers, but readily penetrated (maximum depth of 50 μm) into the scaffold of large diameter fibers. Alkaline phosphatase (ALP) assays showed that MG63 cells differentiated on scaffolds made from both diameter fibers. In longer t

Who reads Fiber Diameters Control Osteoblastic Cell Migration and Differentiation in Electrospun Gelatin?

Author

Kristin Sisson; Chu Zhang; Mary C. Farach-Carson; D. Bruce Chase; John F. Rabolt

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1549-3296)

Published

2010

Language

EN

Field

Engineering (Physical Sciences)