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Isolation and analysis of cell wall material from beeswing wheat bran (Triticum aestivum) by Stephen G. Ring; Robert R. Selvendran is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abtict -Cell wall material (CWM) isolated from beeswing wheat bran contains 66% carbohydrate, 12% Klason lignin. 6% protein and 4% ash. The relative proportions of sugars in the CWM are arabinose 34%. xylose 26%, galactose 2%, glucose 32% and uranic acid 6%. The uranic acid was shown to consist of glucuronic acid and its 4-O-Me analogue in the ratio 1.8 : 1. Partial acid hydrolysis of the CWM yielded neutral sugars and a uranic acid fraction. The latter was shown to contain Glc p A-(l-2)-Xyl p and Glc p A-(l-2)-0-Xyl p-(I -t4)-Xyl p and their 4-O-Me substituted uranic acid analogues. Methylation analysis of the whole CWM and partially degraded methylated CWM revealed the nature of the constituent glycosidic linkages. From the combined evidence we infer that the major structural features of the non-cellulosic polysaccharides are a linear chain of xylopyranose units joined by (1 -+4)-linkages, and arabinofuranose, xylose, galactose (and uranic acid) end groups, which in at least some of the polysaccharides. are attached directly by (1+2)-and/or (l-3)linkages to the xylan chain. The CWM has been fractionated by successive extractions with water at 80". 0.2 M (NH,),C,O, at 80". Na c

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Author

Stephen G. Ring; Robert R. Selvendran

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0031-9422)

Published

1980

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)