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A Novel Approach for a Non Competitive Capillary Electrophoresis Immunoassay with Laser-induced Fluorescence Detection for the Determination of Human Serum Albumin by C. Giovannoli; L. Anfossi; C. Baggiani; G. Giraudi is a Chemistry article available to read on EtoBox.

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A new non competitive capillary electrophoresis immunoassay format based on a separation into a capillary modified by analyte immobilisation is described. The injection of an excess of labelled antibody off-line preincubated with the analyte allows the surface capture of the free antibody and the immunocomplex detection. It was developed using the human serum albumin (HSA) as analyte, two FITC-labelled antibodies and a HSA covalently linked capillary. Two calibration curves with good run-to-run reproducibility and LOD -respectively 14.0 nM for the FITC-polyclonal antibody and 9.0 nM for the FITC-monoclonal antibody -were achieved. The assay was applied to HSA determination in spiked samples of human urine with acceptable recoveries.

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Author

C. Giovannoli; L. Anfossi; C. Baggiani; G. Giraudi

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2007

Language

EN

Field

Chemistry (Physical Sciences)