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Genetic polymorphism of the gene encoding the outer surface protein A (OspA) of Borrelia burgdorferi by G. Zumstein; R. Fuchs; A. Hofmann; V. Preac-Mursic; E. Soutschek; B. Wilske is a Medicine article available to read on EtoBox.

What is Genetic polymorphism of the gene encoding the outer surface protein A (OspA) of Borrelia burgdorferi about?

The genes coding for the outer surface protein (OspA) of Borrelia burgdorferL the causative agent of Lyme borreliosis have been cloned and sequenced. Two German strains (skin isolate PKo and cerebrospinal fluid isolate PBi) have been analyzed. Using an OspA-specific monoclonal antibody (L32 2E7) for immunological screening of a genomic pUC 18 library of B. burgdorferi strain PKo, an OspA-producing clone was detected and subclones containing the open reading frame were constructed. The gene coding for the OspA protein of B. burgdorferi strain PBi was amplified using polymerase chain reaction (PCR) and cloned in pUCS. The open reading frame of both ospA genes consists of 822 nucleotides corresponding to a protein of 273 amino acids. Both proteins have a calculated molecular mass of 29.6 kDa. Molecular analysis revealed significant differences between each other and to already-published sequences of ospA of B. burgdorferi strains B 3 I, ZS 7 and N40 (the ospA genes of B 31, ZS 7 and N 40 are nearly identical). The deduced amino acid sequences of the OspA protein of strains PKo and PBi showed a homology of 83% to each other and 77% and 80%, respectively, to OspA protein of strain B 31.

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Author

G. Zumstein; R. Fuchs; A. Hofmann; V. Preac-Mursic; E. Soutschek; B. Wilske

Publisher

Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0300-8584)

Published

1992

Language

EN

Field

Medicine (Health Sciences)