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Defence-related gene activation during an incompatible interaction between Stagonospora (Septoria) nodorum and barley (Hordeum vulgare L.) coleoptile cells by Conrad Stevens; Elena Titarenko; John A. Hargreaves; Sarah J. Gurr is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Defence-related gene activation during an incompatible interaction between Stagonospora (Septoria) nodorum and barley (Hordeum vulgare L.) coleoptile cells about?

Two previously unidentified cDNA clones (bsil and bprl-1) were isolated by differential hybridization from a cDNA library of Stagonospora (Septoria) nodorum (Berk) Castellani & E.G. Germano (teleomorph Phaeosphaeria (Leptosphaeria) nodorum (E. Muller) Hedjaroude-challenged barley (Hordeum vulgare L.) coleoptiles, bsil encoded a cysteine-rich protein containing 89 amino acids (aa) with a relative molecular mass (Mr) of 9405. Protein sequence homologies showed that Bsil was very similar to an aluminium-induced protein from wheat and indicated that it was related to the Bowman-Birk-type proteinase inhibitors (BB-PIs). The predicted aa sequence of Bsil contained an N-terminal secretory signal sequence which implied that the protein was exported. The other clone, bprl-1, which was truncated at the 5 t end, encoded a type-1 pathogenesis-related (PR-1) protein. The complete sequence of bprl-1 was obtained after cloning a barley genomic DNA fragment and was shown to encode a basic protein containing 174 aa with a Mr of 18 859. The deduced aa sequence of bprl-1 contained both an N-terminal secretory signal sequence and a charged C-terminal extension. This latter sequence may represent a vac

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Author

Conrad Stevens; Elena Titarenko; John A. Hargreaves; Sarah J. Gurr

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0167-4412)

Published

1996

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)