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Phylogenetic distribution and predominant genotype of the avian infectious bronchitis virus in China during 2008-2009 by Jun Ji; Jingwei Xie; Feng Chen; Dingming Shu; Kejing Zuo; Chunyi Xue; Jianping Qin; Hongmei Li; Yingzuo Bi; Jingyun Ma; Qingmei Xie is a Medicine article available to read on EtoBox.
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## Background The nephropathogenic avian infectious bronchitis (IB) caused unprecedented economic losses to the commercial chicken industry of China in 2008-2009. To investigate the prevalence of nephropathogenic IB in China, eighty IBV isolates from different provinces during 2008-2009 were identified by dwarf embryo test and RT-PCR. ## Results The strains were mostly isolated in winter and spring with a wide age range of IB outbreaks, from 4 to 69 days. By the virus recovery trials, 70/80 of the strains resulted in the deaths or distresses of birds from nephritis. To learn more about the molecular evolutionary characteristics of the circulating field strains, the coding region of major spike 1 (S1) protein gene of these strains was RT-PCR amplified and sequenced. Compared to the published representative strains, nucleotides and amino acids sequence analysis indicated that the S1 genes of these strains and the reference strains displayed homologies ranging from 75.1% to 99.8% and from 73.1% to 99.8% respectively. S1 protein of the major pandemic strains contained 540 or 542 amino acids with the cleavage site of HRRRR or RRFRR. Phylogenetic analysis revealed that recent field isola
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- Author
- Jun Ji; Jingwei Xie; Feng Chen; Dingming Shu; Kejing Zuo; Chunyi Xue; Jianping Qin; Hongmei Li; Yingzuo Bi; Jingyun Ma; Qingmei Xie
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2004-; Springer Science and Business Media LLC (ISSN 1743-422X)
- Published
- 2011
- Language
- EN
- Field
- Medicine (Health Sciences)