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Aggregation of polyA–HIV-1 nucleocapsid protein NCp7 complexes and properties of the aggregates by S.P Stoylov; E Stoylova; R Todorov; P Schmiedel; C Thunig; H Hoffmann; BP Roques; E Le Cam; D Coulaud; E Delain; D Gérard; Y Mély is a Materials Science article available to read on EtoBox.
What is Aggregation of polyA–HIV-1 nucleocapsid protein NCp7 complexes and properties of the aggregates about?
We have previously shown that in solution, depending on solute and solution conditions, an ordered aggregation of polyA-NCp7 complexes takes place. The kinetic behaviour of the polyA(homoribopolynucleotide)-NCp7(human immunodeficiency virus-I nucleocapsid protein) aggregating system was studied at physiological NaCl concentration (150 mM) using quasielastic light scattering measurements. The kinetic curves were fitted to a power law equation. For the aggregate size growth with time at initial concentrations of NCp7 = 0.3 mM and nucleotide-to-protein molar ratios of 17 and 50 we obtained for power exponents of 0.15 and 0.23, respectively. On the basis of the aggregate size at which maxima are observed on the light scattering versus time curves we suggested a method for determining the mechanism of aggregation. Thus we found that in the process of polya -NCp7 aggregation, the growth proceeds by fusion of commensurable in size aggregates. The aggregate size dependence on NCp7 concentration showed critical behaviour. The calculated parameters of the power law (rate of growth n and a, related to the aggregate properties) showed in the critical region (0.2-0.3 mM NCp7) a marked change. E
Who reads Aggregation of polyA–HIV-1 nucleocapsid protein NCp7 complexes and properties of the aggregates?
It is typically read by researchers, students, and practitioners in Materials Science.
- Author
- S.P Stoylov; E Stoylova; R Todorov; P Schmiedel; C Thunig; H Hoffmann; BP Roques; E Le Cam; D Coulaud; E Delain; D Gérard; Y Mély
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0927-7757)
- Published
- 1999
- Language
- EN
- Field
- Materials Science (Physical Sciences)